Description
Protease Inhibitor Tablets, EDTA-free, 20 tablets in vials, each tablet is sufficient for 50mL of extract Storage: Upon receipt store at 4
Introduction
The Sunshinebio Protease Inhibitor Tablets protect proteins from degradation by endogenous proteases released during protein extraction and purification procedures. Each tablet contains potent inhibitors (Table 1) against the major classes of proteases, including aspartic acid, cysteine proteases, serine proteases and, when EDTA is included, metalloproteinases.
Table 1. Protease inhibitors contained in tablets. |
AEBSF | Aprotinin | Bestatin | E64 | Leupeptin | Pepstatin A |
|
Important Product Information
• Sunshinebio Protease Inhibitor Tablets contain broad-spectrum protease inhibitors and no phosphatase inhibitors.
• EDTA inhibits metalloproteases and activities of various other proteins; therefore, empirically determine if EDTA is beneficial.
• EDTA interferes with immobilized metal-chelate affinity chromatography (IMAC) and 2D gel electrophoresis. If needed, remove EDTA by extensive dialysis or desalting before performing such procedures.
• Each tablet can prepare 50mL of 1X solution; however, samples containing particularly high levels of proteases may require a final concentration of 2-3X.
Procedure for Using Pierce Protease Inhibitor Tablet
1. Dispense tablet from vial and place into 50mL of solution.
2. Allow tablet to disperse in liquid for 30 seconds.
3. Vortex to dissolve.
Note: Solution may be slightly hazy when tablet is dissolved.
Troubleshooting
Problem | Possible Cause | Solution |
Poor inhibition of protease activity | Sample contained high protease levels | Dissolve the tablet at a higher concentration |
Tablet does not provide sufficient protection against a particular protease family | Inhibitor for a specific family was not a cocktail component | Add individual inhibitors for the specific protease to the samples |
A specific inhibitor was not at the needed concentration | Dissolve the tablet at a higher concentration |
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