Description ：
Protease Inhibitor Tablets: 2 tablets in vials, each tablet is sufficient for 10mL of extract
Storage: Upon receipt store at 4
Introduction ：
The Sunshinebio Protease Phosphatase Inhibitor Tablets protect phosphoproteins from serine phosphatases, threonine phosphatases and protein tyrosine phosphatases (PTPs). Each tablet contains a mixture of four broad specificity inhibitors, including sodium fluoride, sodium orthovanadate, sodium pyrophosphate and β-glycerophosphate. Tablets are conveniently packaged in vials, stable at 4°C and suitable for use with any cell or tissue extract.
Important Product Information
• The Sunshinebio Phosphatase Inhibitor Tablets do not contain protease inhibitors. If necessary, use a broad-spectrum protease inhibitor, such as Sunshinebio Protease Inhibitor Tablets (Product No. PIT8665,PIT8666), in addition to the phosphatase inhibitor tablets. Alternatively, use the combined Sunshinebio Protease and Phosphatase Inhibitor Tablets (Product No.PIT8668, PIT88669).
• The Sunshinebio Inhibitor Tablets can interfere with downstream applications, including certain immobilized metal-chelate affinity chromatography methods. For incompatible procedures, dialyze or desalt sample to effectively remove inhibitors from cell extracts .
• The Sunshinebio Phosphatase Inhibitor Tablets can prepare 10mL of 1X solution; however, samples containing particularly high levels of phosphatase activity may require a final concentration of 2-3X.
• The Sunshinebio Phosphatase Inhibitor Tablets are compatible with Sunshinebio Cell Lysis Reagents.
Procedure for Using Sunshinebio Phosphatase Inhibitor Tablet
1. Dispense from vial and place into 10mL of solution.
2. Allow tablet to disperse in liquid for 30 seconds.
3. Vortex to dissolve.
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