SNU-387 was derived in 1990 by J.-G. Park and associates from a primary hepatocellular carcinoma taken from a Korean patient who had been treated by transcatheter arterial embolization with lipoidol plus a combination of doxorubicin and mitomycin-C. Tumor cells were initially cultured in ACL-4 medium supplemented with 5% heat-inactivated fetal bovine serum. After establishment, cultures were maintained in RPMI 1640 supplemented with 10% heat inactivated fetal bovine serum.
产品品牌:博士德BOSTER
产品名称:人肝胆管癌细胞;RBE
货号:AR0009
产品货号:CX0352
产品名称:SNU-387 (人肝癌细胞)
STR鉴定
种属来源:人
年龄(性别) 女:41岁
组织来源:肝癌
细胞形态:皮细胞样
细胞类型:肿瘤细胞
肿瘤类型:肝癌细胞
背景描述:SNU-387 was derived in 1990 by J.-G. Park and associates from a primary hepatocellular carcinoma taken from a Korean patient who had been treated by transcatheter arterial embolization with lipoidol plus a combination of doxorubicin and mitomycin-C. Tumor cells were initially cultured in ACL-4 medium supplemented with 5% heat-inactivated fetal bovine serum. After establishment, cultures were maintained in RPMI 1640 supplemented with 10% heat inactivated fetal bovine serum.
生长特性:贴壁细胞
培养基:RPMI-1640(PYG0006)+10%FBS(PYG0001/PYG0109)+1%P/S(PYG0016)
倍增时间:61 hours (PubMed=7543080)
生物安全等级:2
推荐传代比例:1:3-1:6
推荐换液频率:2~3次/周
冻存条件:90%FBS+10%DMSO
培养条件 气相:空气,95%;CO2,5% 温度:37℃
备注:本库的细胞系(株)仅用于科研工作,未经许可不得用于其他目的。使用者不得将本库细胞系(株)转让给第三者。